User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/03: Difference between revisions
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== | ==12/03/12== | ||
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* Assembly: KAH111 + C-His expression vector (trouble shooting for Behzad) | * Assembly: KAH111 + C-His expression vector (trouble shooting for Behzad) | ||
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{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
| || 1 || 2 | | || 1 || 2 | ||
|- | |- | ||
| Insert DNA || 1.0 || --- | | Insert DNA || 1.0 || --- | ||
|- | |- | ||
| Vector DNA || 0.5 || 0.5 | | Vector DNA || 0.5 || 0.5 | ||
|- | |- | ||
| 2x lgn buf (Roche) || 5.0 || 5.0 | | 2x lgn buf (Roche) || 5.0 || 5.0 | ||
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 | | T4 ligase (NEB) || 1.0 || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || 2.5 || 3.5 | | dH<sub>2</sub>O || 2.5 || 3.5 | ||
|- | |- | ||
| || 10 μL || 10 μL | | || 10 μL || 10 μL | ||
|} | |} | ||
Revision as of 19:40, 3 December 2012
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12/03/12
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