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 Karmella's BioBrick Cloning
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12/03/12
- Assembly: KAH111 + C-His expression vector (trouble shooting for Behzad)
Assemblies
- KAH111/C-His: KAH111/(E/P)/1123 + C-His/(E/P)/3600
- Measure conc.'s
- Behzad: KAH111 = 15 ng/μL
- Behzad: C-His = 57 ng/μL
| Ligation |
Plate results (lig : neg crtl) mm/dd/yy
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| 1. KAH111(E/P)/1123, 16 ng + C-His(E/P)/3600, 25 ng |
new BioBrick #:1 (Pick #)
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| 2. C-His(E/P)/ 25 ng |
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1 |
2
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| Insert DNA |
1.0 |
---
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| Vector DNA |
0.5 |
0.5
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| 2x lgn buf (Roche) |
5.0 |
5.0
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| T4 ligase (NEB) |
1.0 |
1.0
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| dH2O |
2.5 |
3.5
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10 μL |
10 μL
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Karmella's BioBrick Cloning
