User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/10: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span>
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Latest revision as of 22:19, 26 September 2017

Karmella's BioBrick Cloning Main project page
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12/10/12

  • Retransformation: pSB1A3 (RFP) plasmid from Vi (originally from Rene)


Retransformation

  1. 0.5 μL pSB1A3 (RFP) + 10.0 μL H2O
  2. 10.0 μL H2O (neg. ctrl.)


  • Grow for ~8 hours
  • Pick two (tiny) colonies; grow in 2 mL LB/Amp overnight
  • Return plate to 37 °C (overnight)


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