User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/11: Difference between revisions
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==12/11/12== | ==12/11/12== | ||
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* Note: colonies from retransformation of pSB1A3-RFP look red | |||
* Assembly: KAH111 + C-His (trouble shooting for Behzad, start from plasmids) | * Assembly: KAH111 + C-His (trouble shooting for Behzad, start from plasmids) | ||
* Minipreps: Rene's pSB1A3-RFP (2); cut with X/S for Brady and Vi's Gibson cloning | * Minipreps: Rene's pSB1A3-RFP (2); cut with X/S for Brady and Vi's Gibson cloning | ||
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'''Minipreps'''<br> | '''Minipreps'''<br> | ||
* pSB1A3-RFP (retransformation from 12/10/12) | * pSB1A3-RFP (retransformation from 12/10/12) | ||
* Note: pellets are red | |||
* Concentrations: (1) 260 = , 260/280 = , '''ng/μL = '''; (2) 260 = , 260/280 = , '''ng/μL = '''; | * Concentrations: (1) 260 = , 260/280 = , '''ng/μL = '''; (2) 260 = , 260/280 = , '''ng/μL = '''; | ||
* Digest 10 μL of each with X/S for Gibson cloning (Brady and Vi) | * Digest 10 μL of each with X/S for Gibson cloning (Brady and Vi) |
Revision as of 16:38, 11 December 2012
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12/11/12
Assemblies
Minipreps
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