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Karmella's BioBrick Cloning
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01/02/13
- Gibson assembly: order primers for vector pSB1A3
- Golden Gate assembly: order primers for pSB1A3 and Brady's parts
- Order enzyme for Golden gate assembly:
Gibson Assembly design
- Retry assembly "hPCD-BL01" (see [1]). Make sure primer sequence overlaps with Brady's external sequences:
- BBP-hPCD fwd: gaattcgcggccgcttctaga-tggagctttcagcggtggg (first 21 = BioBrick prefix)
- BL01-BBS rev: ctgcagcggccgctactagt-gccaggatcccccgagcccc (first 20 = BioBrick suffix)
Primers: Golden Gate
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