User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/05: Difference between revisions
From OpenWetWare
Line 48: | Line 48: | ||
* 72°C, 3 min. | * 72°C, 3 min. | ||
* 4°C, ∞ | * 4°C, ∞ | ||
'''Measure DNA Concentrations''' | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | OD 260 | |||
| bgcolor=#cfcfcf | 260/ 280 | |||
| bgcolor=#cfcfcf | ng/μL | |||
|- | |||
| G1 V0120 || 0.108 || 1.801 || 108.375 | |||
|- | |||
| G2 pSB1A3 || 0.101 || 1.849 || 100.553 | |||
|- | |||
| G3 hPCD || 0.034 || 1.876 || 33.586 | |||
|- | |||
| G4 BL01 || 0.113 || 1.828 || 112.79 | |||
|} | |||
Line 88: | Line 105: | ||
* 72°C, 3 min. | * 72°C, 3 min. | ||
* 4°C, ∞ | * 4°C, ∞ | ||
'''Measure DNA Concentrations''' | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | OD 260 | |||
| bgcolor=#cfcfcf | 260/ 280 | |||
| bgcolor=#cfcfcf | ng/μL | |||
|- | |||
| gg1 V0120 || 0.099 || 1.834 || 98.549 | |||
|- | |||
| gg2 pSB1A3 || 0.068 || 1.758 || 67.725 | |||
|- | |||
| gg3 hPCD || 0.022 || 1.72 || 22.485 | |||
|- | |||
| gg4 BL01 || 0.056 || 1.707 || 55.883 | |||
|} | |||
Revision as of 16:37, 5 January 2013
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
01/05/13
Gibson Assembly
Measure DNA Concentrations
Golden Gate Assembly
PCR
|