User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/06: Difference between revisions
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# pSB1A3, 6.0 μL 1/4x Gibson | # pSB1A3, 6.0 μL 1/4x Gibson | ||
* Add DNA to 50 μL DH5α-Turbo; ice/ 10 min. | |||
* Plate on 100 μg/mL amp. | |||
Revision as of 17:38, 6 January 2013
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01/06/13
Gibson Assembly results
PCR
Conclusion: looks like primer dimer. Re-do the transformation with 3x more DNA (from yesterday, stored at -20°C)
Transformation
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