User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/09: Difference between revisions
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* Use gg2 (pSB1A3), gg3, gg4; not gg1 (V0120) since it has BsmBI sites | * Use gg2 (pSB1A3), gg3, gg4; not gg1 (V0120) since it has BsmBI sites | ||
Dilutions | * Dilutions | ||
{| {{table}} border="1" cellspacing="3" <!-- | {| {{table}} border="1" cellspacing="3" <!-- Dilution table --> | ||
|- valign="top" | |- valign="top" | ||
| bgcolor=#cfcfcf | DNA | | bgcolor=#cfcfcf | DNA | ||
| bgcolor=#cfcfcf | length | | bgcolor=#cfcfcf | length (bp) | ||
| bgcolor=#cfcfcf | ng/μL | | bgcolor=#cfcfcf | ng/μL | ||
| bgcolor=#cfcfcf | Vol. | | bgcolor=#cfcfcf | Vol. | ||
| Line 32: | Line 32: | ||
|} | |} | ||
* Golden Gate Reactions | |||
{| {{table}} border="1" cellspacing="3" <!-- Golden Gate Rxn. table --> | |||
|- valign="top" | |||
| Reagent || 1 || 2 | |||
|- | |||
| gg2 pSB1A3 || 1.0 || 1.0 | |||
|- | |||
| gg3 hPCD || 1.0 || --- | |||
|- | |||
| gg4 BL01 || 1.0 || --- | |||
|- | |||
| 10x ligase buffer || 1.0 || 1.0 | |||
|- | |||
| NEB T4 lgase || 0.25 || 0.25 | |||
|- | |||
| BsmBI || 0.5 || 0.5 | |||
|- | |||
| dH<sub>2</sub>O || 5.25 || 7.25 | |||
|- | |||
| || 10.0 || 10.0 | |||
|} | |||
Revision as of 18:46, 9 January 2013
 Karmella's BioBrick Cloning
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01/09/13
Golden Gate Assembly
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