User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/10: Difference between revisions
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PCR | PCR | ||
* 95°C, 3 min. | * 95°C, 3 min. | ||
* [95°C, | * [95°C, 30 sec.; 57°C, 30 sec.; 72°C, 1 min.] x35 | ||
* 72°C, 3 min. | * 72°C, 3 min. | ||
* 4°C, ∞ | * 4°C, ∞ | ||
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Dilutions | Dilutions: to final concentration of 20 fmol/μL | ||
{| {{table}} border="1" cellspacing="3" <!-- Dilution table --> | {| {{table}} border="1" cellspacing="3" <!-- Dilution table --> | ||
|- valign="top" | |- valign="top" |
Revision as of 21:42, 18 January 2013
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01/10/13
Violacein
Golden gate trial 1
Golden gate trial 2
PCR
Thermal cycling
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