User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/11
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| - | '''Minipreps'''<br> | + | '''Golden gate trial 1, Minipreps'''<br> |
* Check with E/P digests | * Check with E/P digests | ||
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| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1-4. hPCD-BL01/pSB1A3 = 2686, 2000 | | rowspan="7" | <u>Expected:</u><br>1-4. hPCD-BL01/pSB1A3 = 2686, 2000 | ||
| - | | rowspan="7" | [[Image: | + | | rowspan="7" | [[Image:KAH011113_gel1.jpg|200px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] |
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| DNA(plasmid) || 3.0 μL | | DNA(plasmid) || 3.0 μL | ||
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| dH<sub>2</sub>O || 8.5 | | dH<sub>2</sub>O || 8.5 | ||
| - | |- | + | |- valign="top" |
| - | | || 15 μL | + | | || 15 μL |
|} | |} | ||
| + | * --> 37°C/ ~15 min. | ||
* None of the digests show both expected bands; probably just vector | * None of the digests show both expected bands; probably just vector | ||
Current revision
 Karmella's BioBrick Cloning
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01/11/13
Golden gate trial 1, Minipreps
Golden gate assembly trial 2
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