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Karmella's BioBrick Cloning
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01/11/13
- Golden Gate assembly trial 1: mini preps/ digests
- Golden gate assembly trial 2: transformation
Minipreps
Reagent
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Volume
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Expected: 1-4. hPCD-BL01/pSB1A3 = 2686, 2000
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15 μL/lane; 1% agarose; Ladder
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DNA(plasmid) |
3.0 μL
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10X buffer |
1.5
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EcoRI |
1.0
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PstI |
1.0
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dH2O |
8.5
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15 μL
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- None of the digests show both expected bands; probably just vector
Golden gate assembly trial 2
- Add 10.0 μL assembly reaction to 50.0 μL DH5α-Turbo; ice/ 5 min.; plate on amp agar
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