User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/11

From OpenWetWare

< User:Karmella Haynes | Notebook | BioBrick cloning | 2013 | 01
Revision as of 18:16, 11 January 2013 by Karmella Haynes (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Karmella's BioBrick Cloning Main project page
Previous entry      Next entry

01/11/13

  • Golden Gate assembly trial 1: mini preps/ digests
  • Golden gate assembly trial 2: transformation


Golden gate trial 1, Minipreps

  • Check with E/P digests
Reagent Volume Expected:
1-4. hPCD-BL01/pSB1A3 = 2686, 2000 		Hover name
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL
  • --> 37°C/ ~15 min.
  • None of the digests show both expected bands; probably just vector


Golden gate assembly trial 2

  • Add 10.0 μL assembly reaction to 50.0 μL DH5α-Turbo; ice/ 5 min.; plate on amp agar



Retrieved from "http://www.openwetware.org/wiki/User:Karmella_Haynes/Notebook/BioBrick_cloning/2013/01/11"
Personal tools