User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/15: Difference between revisions
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| gg3 hPCD || 1.0 || --- || 1.0 || --- || 1.0 || --- | | gg3 hPCD || 1.0 || --- || 1.0 || --- || 1.0 || --- | ||
|- | |- | ||
| gg4 BL01 || 1.0 || --- | | gg4 BL01 || 1.0 || --- || 1.0 || --- || 1.0 || --- | ||
|- | |- | ||
| 10x ligase buffer || 1.0 || 1.0 | | 10x ligase buffer || 1.0 || 1.0|| 1.0 || 1.0|| 5.0 || 5.0 | ||
|- | |- | ||
| NEB T4 lgase || 0.25 || 0.25 | | NEB T4 lgase || 0.25 || 0.25 || 0.25 || 0.25 || 0.25 || 0.25 | ||
|- | |- | ||
| BsmBI || 0.5 || 0.5 | | BsmBI || 0.5 || 0.5 || 0.5 || 0.5 || 0.5 || 0.5 | ||
|- | |- | ||
| dH<sub>2</sub>O || 5.25 || 7.25 | | dH<sub>2</sub>O || 5.25 || 7.25 || 5.25 || 7.25 || 1.25 || 3.25 | ||
|- | |- | ||
| || 10.0 || 10.0 | | || 10.0 || 10.0 || 10.0 || 10.0 || 10.0 || 10.0 | ||
|} | |} | ||
Revision as of 18:07, 16 January 2013
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01/15/13
Golden Gate Assembly optimization
1, 2. Promega - hPCD+BL01+pSB1A3
Thermal cycler
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