User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/16

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01/16/13

  • Golden Gate trial 3: results
  • Cultures: golden gate trial 3, 8 total


Golden Gate assembly trial 3

  • None of the DH5α-Turbo quick transformations had any colonies
  • Promega and Roche ligation buffer trials gave ratios of ~5:1 (assembly : vector only)
  • Pick 4 colonies from the Promega BL21 plate (#2) and the Roche BL21 plate (#10)



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