User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/17: Difference between revisions
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* Transferring inserts from V0120 to pSB1A3 is probably a good idea since several internal BsmBI sites might destroy any template background | * Transferring inserts from V0120 to pSB1A3 is probably a good idea since several internal BsmBI sites might destroy any template background | ||
* Use BsmBI Type IIS method to assemble all the things! | * Use BsmBI Type IIS method to assemble all the things! | ||
1/23/13 Sequencing confirmation (DNASU) | |||
# ggPro-1, VF2 - correct sequence, scarless junction between hPCD and BL01 | |||
# ggPro-1, VR - correct sequence for SP1AB of BL01 | |||
# ggPro-2, VF2 - matches ggPro-1 forward (BLAST) | |||
# ggPro-2, VR - matches ggPro-1 reverse (BLAST) | |||
# ggPro-4, VF2 - matches ggPro-1 forward (BLAST) | |||
#ggPro-4, VR - matches ggPro-1 reverse (BLAST) | |||
Revision as of 13:06, 3 March 2013
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01/17/13
Minipreps
Conclusions
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