User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/19: Difference between revisions
From OpenWetWare
(7 intermediate revisions by the same user not shown) | |||
Line 16: | Line 16: | ||
* Note: made fresh 10x annealing buffer | * Note: made fresh 10x annealing buffer | ||
# | # KAH3/MV2(X) + K-XbaI-NLS-his-stop | ||
* | * K-XbaI-NLS-6his-st 1 | ||
* | * K-XbaI-NLS-6his-st 2 | ||
* | * K-XbaI-NLS-6his-st 3 | ||
* | * K-XbaI-NLS-6his-st 4 | ||
{| | {| {{table}} <!-- Oligo annealing rxn table --> | ||
| DNA (oligos, 100 μM) || 4x 3 μL ea. = 12.0 | | DNA (oligos, 100 μM) || 4x 3 μL ea. = 12.0 | ||
|- | |- | ||
Line 32: | Line 32: | ||
|} | |} | ||
Thermal | Thermal Cycling (try new thermal cycling approach)<br> | ||
Multigene Optimax Thermal Cycler | Multigene Optimax Thermal Cycler | ||
* 99.9 °C, 10 min. | * 99.9 °C, 10 min. | ||
* [99.9°C, temp. increment -2.0°C, 1 min.; | * [99.9°C, temp. increment -2.0°C, 1 min.; 90.0°C, temp. increment -2.0°C, 1 min.] x35 | ||
* 25°C, ∞ | * 25°C, ∞ | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 14:17, 22 March 2013
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||
03/19/13
Oligo annealing
Thermal Cycling (try new thermal cycling approach)
|