User:Karmella Haynes/Notebook/BioBrick cloning/2013/03/19: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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* Note: made fresh 10x annealing buffer | * Note: made fresh 10x annealing buffer | ||
# | # KAH3/MV2(X) + K-XbaI-NLS-his-stop | ||
* | * K-XbaI-NLS-6his-st 1 | ||
* | * K-XbaI-NLS-6his-st 2 | ||
* | * K-XbaI-NLS-6his-st 3 | ||
* | * K-XbaI-NLS-6his-st 4 | ||
{| | {| {{table}} <!-- Oligo annealing rxn table --> | ||
| DNA (oligos, 100 μM) || 4x 3 μL ea. = 12.0 | | DNA (oligos, 100 μM) || 4x 3 μL ea. = 12.0 | ||
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Multigene Optimax Thermal Cycler | Multigene Optimax Thermal Cycler | ||
* 99.9 °C, 10 min. | * 99.9 °C, 10 min. | ||
* [99.9°C, temp. increment -2.0°C, 1 min.; | * [99.9°C, temp. increment -2.0°C, 1 min.; 90.0°C, temp. increment -2.0°C, 1 min.] x35 | ||
* 25°C, ∞ | * 25°C, ∞ | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Latest revision as of 22:33, 26 September 2017
Karmella's BioBrick Cloning | Main project page Previous entry Next entry | ||||||||
03/19/13
Oligo annealing
Thermal Cycling (try new thermal cycling approach)
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