The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Karmella's BioBrick Cloning
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
|
03/19/13
- Oligo annealing for new mammalian vector based on MV9
Oligo annealing
- Note: made fresh 10x annealing buffer
- MV6(X/S) + K-XbaI-NLS-his-stop
- K-XbaI-NLS-his-st 1
- K-XbaI-NLS-his-st 2
- K-XbaI-NLS-his-st 3
- K-XbaI-NLS-his-st 4
DNA (oligos, 100 μM) |
4x 3 μL ea. = 12.0
|
10x annealing buffer |
2.0
|
dH2O |
6.0
|
|
20 μL
|
Thermal Cycling (try new thermal cycling approach)
Multigene Optimax Thermal Cycler
- 99.9 °C, 10 min.
- [99.9°C, temp. increment -2.0°C, 1 min.; 90.0°C, temp. increment -2.0°C, 1 min.] x35
- 25°C, ∞
Vector Digest
|