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 Karmella's BioBrick Cloning
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04/01/13
MV9 clones
- Picked colonies from last cloning attempt. Streaked on LB amp agar.
- Grew up 2 mL cultures overnight and did minipreps.
Minipreps
- Check with S/X digests
- S present in upstream cloning sites, X inside the new insert
| Reagent
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Volume
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Expected:
1-4. MV9 = 4531, 80
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15 μL/lane; 1% agarose; Ladder
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| DNA(plasmid) |
3.0 μL
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| 10X buffer |
1.5
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| EcoRI |
1.0
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| PstI |
1.0
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| dH2O |
8.5
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15 μL --> 37°C/ ~15 min.
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- Result is unclear for #1. Submit for sequencing.
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Karmella's BioBrick Cloning
