User:Karmella Haynes/Notebook/BioBrick cloning/2013/08/29: Difference between revisions
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| NEB BsmBI || 0.5 || 0.5 || 0.5 || 0.5 | | NEB BsmBI || 0.5 || 0.5 || 0.5 || 0.5 | ||
|- | |- | ||
| dH<sub>2</sub>O || 5.25 || 7.25 || 5 | | dH<sub>2</sub>O || 5.25 || 7.25 || 4.5 || 6.5 | ||
|- | |- | ||
| || 10.0 || 10.0 || 10.0 || 10.0 | | || 10.0 || 10.0 || 10.0 || 10.0 |
Revision as of 13:40, 29 August 2013
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08/29/13
Golden Gate
1, 2. 0.25 μL T4 ligase, hPCD+BL01+pSB1A3 or pSB1A3
Note: *DNA is 20 fmol/μL GG Thermal cycling
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