User:Karmella Haynes/Notebook/BioBrick cloning/2013/08/29: Difference between revisions
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{| {{table}} border="1" cellspacing="3" <!-- Golden Gate Rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Golden Gate Rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
| Reagent || Original protocol | | Reagent || Original protocol || Original protocol || More ligase || More ligase | ||
|- | |- | ||
| gg2 pSB1A3* || 1.0 || 1.0 || 1.0 || 1.0 || | | gg2 pSB1A3* || 1.0 || 1.0 || 1.0 || 1.0 || |
Revision as of 13:37, 29 August 2013
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08/29/13
Golden Gate
1, 2. 0.25 μL T4 ligase, hPCD+BL01+pSB1A3 or pSB1A3
Note: *DNA is 20 fmol/μL GG Thermal cycling
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