User:Karmella Haynes/Notebook/BioBrick cloning/2013/08/29: Difference between revisions
From OpenWetWare
Line 29: | Line 29: | ||
| Reagent || Original protocol || Original protocol || More ligase || More ligase | | Reagent || Original protocol || Original protocol || More ligase || More ligase | ||
|- | |- | ||
| gg2 pSB1A3* || 1.0 || 1.0 || 1.0 || 1.0 | | gg2 pSB1A3* || 1.0 || 1.0 || 1.0 || 1.0 | ||
|- | |- | ||
| gg3 hPCD* || 1.0 || --- || 1.0 || --- | | gg3 hPCD* || 1.0 || --- || 1.0 || --- | ||
|- | |- | ||
| gg4 BL01* || 1.0 || --- || 1.0 || --- | | gg4 BL01* || 1.0 || --- || 1.0 || --- | ||
|- | |- | ||
| 10x Promega ligase buffer || 1.0 || 1.0|| 1.0 || 1.0 | | 10x Promega ligase buffer || 1.0 || 1.0|| 1.0 || 1.0 | ||
|- | |- | ||
| NEB T4 ligase || 0.25 || 0.25 || 1.0 || 1.0 | | NEB T4 ligase || 0.25 || 0.25 || 1.0 || 1.0 | ||
|- | |- | ||
| NEB BsmBI || 0.5 || 0.5 || 0.5 || 0.5 | | NEB BsmBI || 0.5 || 0.5 || 0.5 || 0.5 | ||
|- | |- | ||
| dH<sub>2</sub>O || 5.25 || 7.25 || 5.25 || 7.25 | | dH<sub>2</sub>O || 5.25 || 7.25 || 5.25 || 7.25 | ||
|- | |- | ||
| || 10.0 || 10.0 || 10.0 || 10.0 | | || 10.0 || 10.0 || 10.0 || 10.0 | ||
|} | |} | ||
Note: *DNA is 20 fmol/μL | Note: *DNA is 20 fmol/μL |
Revision as of 13:38, 29 August 2013
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||
08/29/13
Golden Gate
1, 2. 0.25 μL T4 ligase, hPCD+BL01+pSB1A3 or pSB1A3
Note: *DNA is 20 fmol/μL GG Thermal cycling
|