User:Karmella Haynes/Notebook/BioBrick cloning/2013/08/29: Difference between revisions
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* Golden Gate Assembly optimization - increase ligase amount; redo hPCD assembly | * Golden Gate Assembly optimization - increase ligase amount; redo last successful hPCD assembly | ||
Revision as of 13:38, 29 August 2013
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08/29/13
Golden Gate
1, 2. 0.25 μL T4 ligase, hPCD+BL01+pSB1A3 or pSB1A3
Note: *DNA is 20 fmol/μL GG Thermal cycling
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