User:Karmella Haynes/Notebook/BioBrick cloning/2013/09/05

From OpenWetWare

< User:Karmella Haynes | Notebook | BioBrick cloning | 2013 | 09(Difference between revisions)
Jump to: navigation, search
(09/05/13)
Current revision (11:32, 7 September 2013) (view source)
(09/05/13)
 
Line 13: Line 13:
----
----
'''Liquid cultures'''<br>
'''Liquid cultures'''<br>
-
* CMV, in V0120 vector
+
* CMV, in V0120 vector (BBa_J176027) -- not 176017!
** Transformation was done on [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/BioBrick_cloning/2013/09/05 9/04/13]
** Transformation was done on [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/BioBrick_cloning/2013/09/05 9/04/13]
** Result for Plate 1: well-defined colonies and some micro-colonies, probably overgrowth, but still usable
** Result for Plate 1: well-defined colonies and some micro-colonies, probably overgrowth, but still usable

Current revision

Karmella's BioBrick Cloning Main project page
Previous entry      Next entry

09/05/13

  • Grow liquid cultures for CMV/V0120



Liquid cultures

  • CMV, in V0120 vector (BBa_J176027) -- not 176017!
    • Transformation was done on 9/04/13
    • Result for Plate 1: well-defined colonies and some micro-colonies, probably overgrowth, but still usable
    • Result for Plate 2 (negative control): no growth at all, so overall, transformation worked as expected


List of Samples for Liquid Cultures

  1. Culture 1 = Colony 1 from plate 1, CMV, ampicillin
  2. Culture 2 = Colony 2 from plate 1, same



Personal tools