09/06/13
- Miniprep - CMV
- Restriction digest - CMV
- Sequencing - CMV [cloning bootcamp demo]
Results
- CMV/V0120
- Culture innoculated on 9/05/13
- Results for liquid cultures: (1) grew, (2) grew
- Results for streak plate: (1) grew well, (2) hardly any growth
List of Samples for Minprep & Analysis
- Sample 1 = CMV/V0120 colony 1; BBa_J176027; part = 588 bp; vector = 3200
- Sample 2 = CMV/V0120 colony 2; same
- Sample 3 = CMV/V0120 (control DNA); BBa_J176027; part = 588 bp; vector = 3200
DNA Concentration Data
Plasmid |
OD260 |
OD260/280 |
ng/μL
|
1. Plasmid 1 |
0.108 |
1.841 |
108.321
|
2. Plasmid 2 |
0.007 |
1.75 |
6.60
|
- Streak #2 did not grow well. Culture might be empty bacteria. Use mini prep 1 for other steps.
Restriction Digest Table
- Checked plasmid minipreps with EcoRI/PstI digests
Reagent
|
Volume
|
Expected: 1. Plasmid 1 = insert size, vector size 2. Plasmid 2 = insert size, vector size
|
15 μL/lane; 1% agarose; Ladder
|
DNA(plasmid) |
3.0 μL
|
10X buffer |
1.5
|
EcoRI |
1.0
|
PstI |
1.0
|
dH2O |
8.5
|
|
15 μL --> 37°C/ 15 min.
|
DNA Sequencing Samples
- Submitted to DNASU on 'date'
- Plasmid 1 - forward primer 'name'
- Plasmid 1 - reverse primer 'name'
- Plasmid 2 - forward primer 'name'
- Plasmid 2 - reverse primer 'name'
|