User:Karmella Haynes/Notebook/BioBrick cloning/2015/01/22: Difference between revisions
From OpenWetWare
Line 37: | Line 37: | ||
Try different cDNA libraries for the ATF2 fragment to account for any mutations or deletions | Try different cDNA libraries for the ATF2 fragment to account for any mutations or deletions | ||
# KAH60 "backbone" (without VP64, includes scars) = 6637: KAH60 F1/ KAH60 R1 | # KAH60 "backbone" (without VP64, includes scars) = 6637: KAH60 F1/ KAH60 R1 | ||
# U2OS C002; ATF2 insert = 906: ATF2_f1/ ATF2_r1 | # U2OS C002, 1:1000 dilution; ATF2 insert = 906: ATF2_f1/ ATF2_r1 | ||
# SKNSH C001; same | # SKNSH C001, 1:1000 dilution; same | ||
# K562 C001; same | # K562 C001, 1:1000 dilution; same | ||
Line 49: | Line 49: | ||
| bgcolor=#cfcfcf | Rxn3 | | bgcolor=#cfcfcf | Rxn3 | ||
| bgcolor=#cfcfcf | Rxn4 | | bgcolor=#cfcfcf | Rxn4 | ||
| rowspan="7" | [[Image:KAH012315_gel.jpg|270px|Hover name]]<br>10 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:KAH012315_gel.jpg|270px|Hover name]]<br>10 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| Template || 0.2 KAH60/pcVN || 1.0 | | Template || 0.2 KAH60/pcVN || 1.0 U2OS cDNA || 1.0 SKNSH cDNA || 1.0 K562 cDNA | ||
|- | |- | ||
| 10 uM fwd primer || 1.0 || 1.0 || 1.0 || 1.0 | | 10 uM fwd primer || 1.0 || 1.0 || 1.0 || 1.0 |
Revision as of 10:36, 27 January 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||
01/22/15
Planning: Gal4 fusion cloning
Program: GOTAQ35cyc
|