User:Karmella Haynes/Notebook/BioBrick cloning/2015/03/26: Difference between revisions
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'''Make PEG solutions''' | '''Make PEG solutions''' | ||
* | * Make 30 mM MgCl2, 50 mL (plus one extra): 1.5 mL 1M MgCl2 + 48.5 molecular bio grade H<sub>2</sub>O | ||
* Make 30% PEG, 50 mL: 1.5 g PEG 8000 + 50 mL 30 mM MgCL2 | |||
* Use this to make other solutions | * Use this to make other solutions | ||
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| rowspan="7" | <!-- [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] --> | | rowspan="7" | <!-- [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] --> | ||
|- | |- | ||
| 30% PEG || | | 30% PEG || 6.0 mL || 5.0 mL || 4.0 mL || 3.0 mL || 2.0 mL | ||
|- | |- | ||
| 30 mM MgCl2 || | | 30 mM MgCl2 || 4.0 mL || 5.0 mL || 6.0 mL || 7.0 mL || 8.0 mL | ||
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|- | |- | ||
| || 10 mL | | || 10 mL |
Revision as of 11:46, 26 March 2015
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03/26/15
PEG precipitation - optimization
Assemblies
Oligo annealing
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