User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/09: Difference between revisions
From OpenWetWare
Line 16: | Line 16: | ||
* '''MV10''' (5191 bp): Template - MV10 plasmid, F - MV10 f1, R - MV10 r1 | * '''MV10''' (5191 bp): Template - MV10 plasmid, F - MV10 f1, R - MV10 r1 | ||
* Gradient PCR to enhance 5 kb band | * Gradient PCR to enhance 5 kb band | ||
# | # 68°C | ||
# | # 67.3°C | ||
# | # 65.9°C | ||
# | # 63.9°C | ||
# | # 61.4°C | ||
# | # 59.6°C | ||
# | # 58.1°C | ||
# | # 57°C | ||
{| {{table}} cellspacing="3" <!-- PCR rxn. table --> | {| {{table}} cellspacing="3" <!-- PCR rxn. table --> | ||
Line 50: | Line 50: | ||
|} | |} | ||
Program: Phusion (block | Program: Phusion (block A) - edited to include 65°C - 55°C annealing gradient | ||
* 98°C, 3 min | * 98°C, 3 min | ||
* 30x[ | * 30x[98°C, 10 sec; 68°C - 57°C, 30 sec; 72°C, 2.5 min] | ||
* 72°C, | * 72°C, 10 min | ||
* 4°C ∞ | * 4°C ∞ | ||
Revision as of 17:56, 9 April 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||
04/09/15
LCR development - Backbone PCR
Program: Phusion (block A) - edited to include 65°C - 55°C annealing gradient
|