User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/17: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
(4 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 35: | Line 35: | ||
| bgcolor=#cfcfcf | Mix (x5) | | bgcolor=#cfcfcf | Mix (x5) | ||
| rowspan="7" | <u>Expected:</u><br>1. AubR = 851<br>2. BjaR = 776<br>3. BraR = 776<br>4. RpaR = 779<br> | | rowspan="7" | <u>Expected:</u><br>1. AubR = 851<br>2. BjaR = 776<br>3. BraR = 776<br>4. RpaR = 779<br> | ||
| rowspan="7" | [[Image:KAH041715_gel1.jpg|200px|Hover name]]<br> | | rowspan="7" | [[Image:KAH041715_gel1.jpg|200px|Hover name]]<br>5 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA(plasmid) || 0.5 μL || --- | | DNA(plasmid) || 0.5 μL || --- | ||
Line 62: | Line 62: | ||
Conclusion | Conclusion | ||
* | * All four worked. Proceed to next step | ||
Line 76: | Line 76: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Rxn1,2 | | bgcolor=#cfcfcf | Rxn1,2 | ||
| rowspan="7" | <u>Expected:</u><br>1,2. MV10 = | | rowspan="7" | <u>Expected:</u><br>1,2. MV10 = 5191 | ||
| rowspan="7" | [[Image:KAH041715_gel2.jpg|150px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:KAH041715_gel2.jpg|150px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
Line 104: | Line 104: | ||
Conclusion | Conclusion | ||
* | * Big improvement. Keep this for potential cloning | ||
* Also try again with higher annealing temp | |||
Latest revision as of 00:55, 27 September 2017
Karmella's BioBrick Cloning | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||
04/17/15
Ryan - Receiver plasmids, assembly
Conclusion
LCR Development
Conclusion
|