User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/22: Difference between revisions
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* Ryan - streak plates & liquid cultures (see 4/21/15) | * Ryan - streak plates & liquid cultures (see 4/21/15) | ||
* Rene - minipreps: g53-2, g54 | * Rene - minipreps: g53-2, g54 | ||
* LCR Development | * LCR Development - assembly attempt #1 | ||
---- | |||
'''LCR - assembly attempt #1''' | |||
* Refer to [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/BioBrick_cloning/2015/04/06 04/06/15] | |||
Column-purify PCR fragments | |||
* Use Qiagen PCR Clean-up, elute and back-elute w/ 30 μL elution buffer | |||
# Gal4DB-mCh (4/22/15) | |||
# ATF2 (4/22/15) | |||
# MV10 (4/17/15) | |||
* Measure conc.'s | |||
{| {{table}} cellspacing="3" <!-- [DNA] table --> | |||
|- bgcolor=#cfcfcf | |||
| Sample || OD260 || 260/280 || ng/μL | |||
|- | |||
| 1. 5'p Gal4DB-mCh PCR || --- || --- || --- | |||
|- | |||
| 2. 5'p ATF2 PCR || --- || --- || --- | |||
|- | |||
| 2. 5'p MV10 PCR || --- || --- || --- | |||
|} | |||
Revision as of 15:57, 22 April 2015
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04/22/15
LCR - assembly attempt #1
Minipreps
Assemblies
Oligo annealing
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