User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/22: Difference between revisions
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* Rene - minipreps & sequencing: g53-2, g54 | * Rene - minipreps & sequencing: g53-2, g54 | ||
* LCR Development - assembly attempt #1 | * LCR Development - assembly attempt #1 | ||
---- | |||
'''Rene - minipreps & sequencing''' | |||
# gRNA53-2 | |||
# gRNA54 | |||
* Sigma GenElute kit | |||
* Elute w/ 75 μL elution sln. | |||
* Measure conc.'s | |||
{| {{table}} cellspacing="3" <!-- [DNA] table --> | |||
|- bgcolor=#cfcfcf | |||
| Sample || OD260 || 260/280 || ng/μL | |||
|- | |||
| 1. g53-2 || 0.186 || 1.863 || 186.1 | |||
|- | |||
| 2. g54 || 0.205 || 1.895 || 205.2 | |||
|} | |||
* Submit to DNASU for sequencing | |||
# g53-2, f (P139) | |||
# g53-2, r (P139) | |||
# g54, f (P139) | |||
# g54, r (P139) | |||
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| Sample || OD260 || 260/280 || ng/μL | | Sample || OD260 || 260/280 || ng/μL | ||
|- | |- | ||
| 1. 5'p Gal4DB-mCh PCR || | | 1. 5'p Gal4DB-mCh PCR || 0.139 || 1.825 || 138.6 | ||
|- | |- | ||
| 2. 5'p ATF2 PCR || | | 2. 5'p ATF2 PCR || 0.191 || 1.828 || 190.8 | ||
|- | |- | ||
| 2. 5'p MV10 PCR || | | 2. 5'p MV10 PCR || 0.066 || 1.716 || 65.9 | ||
|} | |} | ||
Revision as of 17:24, 22 April 2015
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04/22/15
Rene - minipreps & sequencing
LCR - assembly attempt #1
LCR Reactions (PCR fragments / oligo bridges)
Thermocycler: BioRad dual block
Minipreps
Assemblies
Oligo annealing
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