User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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| bgcolor=#cfcfcf | Rxn 1 | | bgcolor=#cfcfcf | Rxn 1 | ||
| bgcolor=#cfcfcf | Rxn 2 | | bgcolor=#cfcfcf | Rxn 2 | ||
| rowspan="7" | [[Image:KAH050115_gel1.jpg| | | rowspan="7" | [[Image:KAH050115_gel1.jpg|200px|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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| DNA (plasmid) || 20.0 || 15.0 | | DNA (plasmid) || 20.0 || 15.0 | ||
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| dH<sub>2</sub>O || 5.0 || 10.0 | | dH<sub>2</sub>O || 5.0 || 10.0 | ||
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| || 30 μL | | || 30 μL || | ||
|} | |} | ||
--> 37°C/ ~30 min. | --> 37°C/ ~30 min. | ||
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| dH<sub>2</sub>O || 9.9 | | dH<sub>2</sub>O || 9.9 | ||
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| || 20 | | || 20 μL | ||
|} | |} | ||
--> 37°C/ 10 min.; 75°C/ 2 min.; [final] = 25 ng/μL | |||
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RESULTS (5/01/15) | |||
* Success! Plate #1 has 50 colonies, neg ctrl has 1 colony | |||
* Pick 4 colonies (50% chance of fwd vs. reverse insertion) | |||
Latest revision as of 00:56, 27 September 2017
Karmella's BioBrick Cloning | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/01/15
Assembly: KAH87 (hPcTF ORF) into MV10
--> 37°C/ ~30 min.
--> 37°C/ 10 min.; 75°C/ 2 min.; [final] = 25 ng/μL
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