User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/15: Difference between revisions
From OpenWetWare
(One intermediate revision by the same user not shown) | |||
Line 64: | Line 64: | ||
| 4. RpaR/MRV(BbsI) || 0.047 || 1.858 || 46.7 | | 4. RpaR/MRV(BbsI) || 0.047 || 1.858 || 46.7 | ||
|- | |- | ||
| 5. | | 5. DBN001 (BsaI) || 0.017 || 2.176 || 16.8 | ||
|} | |} | ||
Line 106: | Line 106: | ||
---- | ---- | ||
''' | '''Cas-tone - minipreps, digests''' | ||
* Sigma GenElute mini kit; elute w/ 75μL elution sln. | |||
* Check with E/S digests - SpeI site (@250) is destroyed and new SpeI site (@844) is gained in successful ligation | * Minipreps | ||
** Sigma GenElute mini kit; elute w/ 75μL elution sln. | |||
** Check with E/S digests - SpeI site (@250) is destroyed and new SpeI site (@844) is gained in successful ligation | |||
# CMV-dCas9-VP64 1-1 | # CMV-dCas9-VP64 1-1 |
Revision as of 12:47, 19 May 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/15/15
Assemblies
--> 37°C/ ~30 min.
Cas-tone - minipreps, digests
--> 37°C/ ~10 min.
|