User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/18: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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| bgcolor=#cfcfcf | Rxn2 | | bgcolor=#cfcfcf | Rxn2 | ||
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| Insert DNA || 2.0 || --- | | Insert DNA || 2.0 || --- | ||
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| Vector DNA || 1.5 || 1.5 | | Vector DNA || 1.5 || 1.5 | ||
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| 2x lgn buf (Roche) || 5.0 || 5.0 | | 2x lgn buf (Roche) || 5.0 || 5.0 | ||
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| T4 ligase (NEB) || 1.0 || 1.0 | | T4 ligase (NEB) || 1.0 || 1.0 | ||
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| dH<sub>2</sub>O || 0.5 || 2.5 | | dH<sub>2</sub>O || 0.5 || 2.5 | ||
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| || 10.0 μL || 10.0 μL | | || 10.0 μL || 10.0 μL | ||
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Latest revision as of 00:58, 27 September 2017
Karmella's BioBrick Cloning | Main project page Previous entry Next entry | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/18/15
Ben - assembly: HPK-CFP into homology arms
Program: Phusion (block B)
Conclusion
Cas-tone Project
Assembly
--> 37°C/ ~30 min.
LabNet OptiMax Thermocycler: AnOlig RD
Dilute the product(s) 1:250
Rene - PCR cloning trial 3
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