User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/18: Difference between revisions
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| || 10.0 μL || 10.0 μL || | | || 10.0 μL || 10.0 μL || | ||
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RESULTS (5/19/15) | |||
* Success! ~100 colonies on ligation plate, 6 on neg ctrl. | |||
* Pick two colonies for 5mL cultures | |||
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RESULTS (5/ | RESULTS (5/19/15) | ||
* | * Success! ~200 colonies on each plate. Pick for 96-well "mass culture" plates | ||
Revision as of 13:02, 19 May 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/18/15
Ben - assembly: HPK-CFP into homology arms
Program: Phusion (block B)
Conclusion
Cas-tone Project
Assembly
--> 37°C/ ~30 min.
LabNet OptiMax Thermocycler: AnOlig RD
Dilute the product(s) 1:250
Rene - PCR cloning trial 3
|