User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/18: Difference between revisions
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| bgcolor=#cfcfcf | Rxn2 | | bgcolor=#cfcfcf | Rxn2 | ||
|- | |- | ||
| Insert DNA || 2.0 || --- | | Insert DNA || 2.0 || --- | ||
|- | |- | ||
| Vector DNA || 1.5 || 1.5 | | Vector DNA || 1.5 || 1.5 | ||
|- | |- | ||
| 2x lgn buf (Roche) || 5.0 || 5.0 | | 2x lgn buf (Roche) || 5.0 || 5.0 | ||
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 | | T4 ligase (NEB) || 1.0 || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || 0.5 || 2.5 | | dH<sub>2</sub>O || 0.5 || 2.5 | ||
|- | |- | ||
| || 10.0 μL || 10.0 μL | | || 10.0 μL || 10.0 μL | ||
|} | |} | ||
Revision as of 13:07, 19 May 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/18/15
Ben - assembly: HPK-CFP into homology arms
Program: Phusion (block B)
Conclusion
Cas-tone Project
Assembly
--> 37°C/ ~30 min.
LabNet OptiMax Thermocycler: AnOlig RD
Dilute the product(s) 1:250
Rene - PCR cloning trial 3
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