User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/21: Difference between revisions
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* Ligations | * Ligations | ||
** > 2:1 ration calculations... | |||
** 433 bp largest insert / 9506 bp vector * 2 * 100 ng vector = 9.0 ng | |||
** 129 bp smallest insert / 9506 bp vector * 2 * 100 ng vector = 2.7 ng | |||
{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
| || 1 || 2 | |- bgcolor="#cfcfcf" valign="top" | ||
| Reagent | |||
| Rxn1-5 | |||
| Rxn6 | |||
| || 1 || 2 | |||
|- | |- | ||
| Insert DNA || ### || --- | | Insert DNA || ### || --- | ||
|- | |- | ||
| Vector DNA || ### || ### | | Vector DNA || ### || ### | ||
|- | |- | ||
| 2x lgn buf (Roche) || ### || ### | | 2x lgn buf (Roche) || ### || ### | ||
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 | | T4 ligase (NEB) || 1.0 || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || ### || ### | | dH<sub>2</sub>O || ### || ### | ||
|- | |- | ||
| || # μL || # μL | | || # μL || # μL | ||
|} | |} | ||
Revision as of 17:27, 21 May 2015
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05/21/15
Cas-tone Project
Thermal cycler: LabNet OptiMax "AnOlig shrt"
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