User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/27: Difference between revisions
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** Melt: continuous acquisition; 95°C, 4 sec; 70°C, 60 sec, -- +0.1°C/ 5 sec (programmed as "6 acquisitions/°C") --> 95°C | ** Melt: continuous acquisition; 95°C, 4 sec; 70°C, 60 sec, -- +0.1°C/ 5 sec (programmed as "6 acquisitions/°C") --> 95°C | ||
** Cooling: 40°C, 30 sec | ** Cooling: 40°C, 30 sec | ||
RESULTS: | |||
# Lu34_AA01 - 12 -- Melt temps 85.1 ± 0.1 °C; additional short peak ~87°C | |||
# Ga34_AA01 - 12 -- Melt temps 85.1 ± 0.1 °C; additional short peak ~87°C | |||
# nTc (3 wells) -- no amplification | |||
* Try using more diluted template (100x) | |||
* Continue by sequencing a few purified PCR products with P215 to find a reference sample (wild type sequence) | |||
Revision as of 15:25, 28 May 2015
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05/27/15
Rene - hi-res melt curve screen on CRISPR clones
RESULTS:
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