User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==05/29/15== | ||
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* David Tze - minpreps & digests | * David Tze - minpreps & digests | ||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. DT001 = | | rowspan="7" | <u>Expected:</u><br>1. DT001 = 2668, 252<br>Empty PLflex = 2668, 95<br>2. DT002 = 2656, 432<br>Empty PLflex4 = 2656, 274<br>3. DT003 = 2668, 252<br>Empty PLrigid = 2668, 95<br>4. DT004 = 2656, 432<br>Empty PLrigid4 = 2656, 275 | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:KAH052915_gel1.jpg|250px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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| DNA(plasmid) || 2.0 μL | | DNA(plasmid) || 2.0 μL | ||
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| || 15 μL --> 37°C/ ~15 min. | | || 15 μL --> 37°C/ ~15 min. | ||
|} | |} | ||
CONCLUSIONS: | |||
# DT001 - success. | |||
# DT002 - success. | |||
# DT003 - success. | |||
# DT004 - success. | |||
* Next steps: | |||
** Sequence verify all clones (extra precaution; linker part is small) | |||
---- | ---- | ||
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* 5-16 - Cas-tone | * 5-16 - Cas-tone | ||
# | # DBN007-1 - VF2 - good | ||
# | # DBN007-1 - VR - good, pSB1A3 terminator appears to be deleted | ||
# | # DBN007-2 - VF2 - good | ||
# | # DBN007-2 - VR - good, pSB1A3 terminator appears to be deleted | ||
# | # H3140-1 - CMVf | ||
# | # H3140-1 - DD123 | ||
# | # H3140-2 - CMVf | ||
# | # H3140-2 - DD123 | ||
# | # H3160-1 - CMVf | ||
# | # H3160-1 - DD123 | ||
# | # H3160-2 - CMVf | ||
# | # H3160-2 - DD123 | ||
# | # H3180-1 - CMVf | ||
# | # H3180-1 - DD123 | ||
# | # H3180-2 - CMVf | ||
# | # H3180-2 - DD123 | ||
# | # H31116-2 - CMVf | ||
# | # H31116-2 - DD123 | ||
# | # H31136-1 - CMVf | ||
# | # H31136-1 - DD123 | ||
Latest revision as of 01:00, 27 September 2017
Karmella's BioBrick Cloning | Main project page Previous entry Next entry | ||||||||||||||||
05/29/15
David Tze - minpreps & digests
CONCLUSIONS:
Sequencing order
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