User:Karmella Haynes/Notebook/BioBrick cloning/2015/05/29: Difference between revisions
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(Autocreate 2015/05/29 Entry for User:Karmella_Haynes/Notebook/BioBrick_cloning) |
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==mm/dd/yy== | ==mm/dd/yy== | ||
<!-- Precede finished items with a checkmark ✓ --> | <!-- Precede finished items with a checkmark ✓ --> | ||
* | * David Tze - minpreps & digests | ||
* | * Ben - sequencing order | ||
* Cas-tone - sequencing order | |||
---- | ---- | ||
''' | '''David Tze - minpreps & digests''' | ||
* Minipreps | * Minipreps | ||
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* Digests | * Digests | ||
** Check with | ** Check with E/S digests; cannot use PstI because PLrigid & PLrigid4 contain PstI sites | ||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. | | rowspan="7" | <u>Expected:</u><br>1. DT001 = size<br>2. DT002 = size<br>3. DT003 = size<br>4. DT004 = size<br>Empty vectors = ~2600, ~400 - 600 | ||
| rowspan="7" | | | rowspan="7" | [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA(plasmid) || 2.0 μL | | DNA(plasmid) || 2.0 μL | ||
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| EcoRI || 1.0 | | EcoRI || 1.0 | ||
|- | |- | ||
| | | SpeI || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || 9.5 | | dH<sub>2</sub>O || 9.5 | ||
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---- | ---- | ||
''' | '''Sequencing orders''' | ||
# DBN007_1 - | |||
Revision as of 14:31, 29 May 2015
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mm/dd/yy
David Tze - minpreps & digests
Sequencing orders
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