User:Karmella Haynes/Notebook/BioBrick cloning/2015/06/18: Difference between revisions
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** 200 ng plasmid + 1 μL 100 μM primer | ** 200 ng plasmid + 1 μL 100 μM primer | ||
** Results: aligned with pUAS-TK-Luc (https://benchling.com/s/Qyqsd7/edit) | ** Results: aligned with pUAS-TK-Luc (https://benchling.com/s/Qyqsd7/edit) | ||
# Lu34_AA01/ P163 - | ** Positions of mutations named relative to the 5' base at target g034 | ||
# Lu34_AA01/ P215 - | # Lu34_AA01/ P163 - wild type | ||
# Lu34_AA02/ P163 - | # Lu34_AA01/ P215 - wild type | ||
# Lu34_AA02/ P215 - | # Lu34_AA02/ P163 - DEL:11-17 | ||
# Ga34_AA01/ P163 - | # Lu34_AA02/ P215 - DEL:11-17 | ||
# Ga34_AA01/ P215 - | # Ga34_AA01/ P163 - wild type | ||
# Ga34_AA02/ P163 - | # Ga34_AA01/ P215 - wild type | ||
# Ga34_AA02/ P215 - | # Ga34_AA02/ P163 - wild type | ||
# Ga34_AA02/ P215 - wild type | |||
* Compare these results to previous qPCR/melt | |||
Revision as of 17:03, 22 June 2015
 Karmella's BioBrick Cloning
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06/18/15
Rene - Minipreps - CRISPR library clones
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