User:Karmella Haynes/Notebook/BioBrick cloning/2015/06/18

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06/18/15

  • Minipreps - CRISPR library clones


Rene - Minipreps - CRISPR library clones

  • Minipreps
    • Sigma GenElute kit, elute w/ 75 μL elution sln.
  1. Lu34_AA01
  2. Lu34_AA02
  3. Ga34_AA01
  4. Ga34_AA02
  • Sequencing - DNASU (Order# 10244)
    • 200 ng plasmid + 1 μL 100 μM primer
    • Results: aligned with pUAS-TK-Luc (https://benchling.com/s/Qyqsd7/edit)
    • Positions of mutations named relative to the 5' base at target g034
  1. Lu34_AA01/ P163 - wild type
  2. Lu34_AA01/ P215 - wild type
  3. Lu34_AA02/ P163 - DEL:11-17
  4. Lu34_AA02/ P215 - DEL:11-17
  5. Ga34_AA01/ P163 - wild type
  6. Ga34_AA01/ P215 - wild type
  7. Ga34_AA02/ P163 - wild type
  8. Ga34_AA02/ P215 - wild type




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