User:Karmella Haynes/Notebook/BioBrick cloning/2015/06/22: Difference between revisions
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*** L34 = Luc14 cells treated with gRNA034 | *** L34 = Luc14 cells treated with gRNA034 | ||
*** G34 = Gal4EED/luc cells treated with gRNA034 | *** G34 = Gal4EED/luc cells treated with gRNA034 | ||
*** 001, 002, 003 = | *** 001, 002, 003 = clone no. | ||
*** A01, A02, etc. = well position in dish or spot on agar array | *** A01, A02, etc. = well position in dish or spot on agar array | ||
Revision as of 16:47, 22 June 2015
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06/22/15
Rene - CRISPR PCR library PCR
1-96. L34_001_A01 - L34_096_H12 (full plate)
Cas-tone - Phusion PCR for H3
Thermal cycler: Bio-Rad - Phusion
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