User:Karmella Haynes/Notebook/BioBrick cloning/2015/08/25: Difference between revisions

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# L34-027, C4, 399
# L34-027, C4, 399
# L34-032, H4, 339
# L34-032, H4, 339
# L34-040, H5, 494
# L34-050, B7, 303
# L34-055, G7, 563
# L34-071, G9, 237
# L34-075, C10, 300
# L34-078, F10, 647




Revision as of 16:13, 26 August 2015

Karmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

08/25/15

  • Cultures - CRISPR library 5 mL cultures


CRISPR library 5 mL cultures

  • Re-grow cultures for clones with failed sequencing runs
  • Note: DNASU sampling from the 96-well block is top to bottom, then left to right
    • A1 = L34-001, A2 = L34-002, A3 = L34-003, etc.
  • Low phred scores (<700) generally corresponded with the lowqual reads observed in Benchling
  • Scrape bacteria from the original spot agar plates; grow as 5 mL culture for miniprep (overnight)

Clone, well (agar spot position), phred score

  1. L34-003, C1, 313
  2. L34-015, G2, 281
  3. L34-027, C4, 399
  4. L34-032, H4, 339
  5. L34-040, H5, 494
  6. L34-050, B7, 303
  7. L34-055, G7, 563
  8. L34-071, G9, 237
  9. L34-075, C10, 300
  10. L34-078, F10, 647


RESULTS (08/26/15)

  • Only four clones grew
  1. L34-032
  2. L34-040
  3. L34-094
  4. L34-096



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