User:Karmella Haynes/Notebook/BioBrick cloning/2015/08/26: Difference between revisions

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Line 29: Line 29:
| Sample || OD260 || 260/280 || ng/μL
| Sample || OD260 || 260/280 || ng/μL
|-
|-
| 1. Digested part (a/b) || --- || --- || ---
| 1. L34-032 || 0.167 || 1.863 || 167.3
|-
|-
| 2. Digested part (c/d) || --- || --- || ---
| 2. L34-040 || 0.026 || 1.908 || 26.4
|-
| 3. L34-094 || 0.032 || 1.901 || 32.4
|-
| 4. L34-096 || 0.142 || 1.859 || 142.4
|}
|}


* Sequencing
* Sequencing: Order 10437
# L34-032, P163
# L34-040 "
# L34-094 "
# L34-096 "




Revision as of 16:53, 26 August 2015

Karmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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08/26/15

  • Minipreps - CRISPR library
  • Cultures - CRISPR library (round 2)


Minipreps - CRISPR library


  • Minipreps
    • Sigma GenElute kit, elute w/ 75 μL elution sln.
  1. L34-032
  2. L34-040
  3. L34-094
  4. L34-096


  • Measure conc.'s
Sample OD260 260/280 ng/μL
1. L34-032 0.167 1.863 167.3
2. L34-040 0.026 1.908 26.4
3. L34-094 0.032 1.901 32.4
4. L34-096 0.142 1.859 142.4
  • Sequencing: Order 10437
  1. L34-032, P163
  2. L34-040 "
  3. L34-094 "
  4. L34-096 "



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