User:Karmella Haynes/Notebook/BioBrick cloning/2015/08/26: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 43: Line 43:
# L34-094 "
# L34-094 "
# L34-096 "
# L34-096 "
----
'''Cultures - CRISPR library (round 2)'''





Revision as of 17:03, 26 August 2015

Karmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

08/26/15

  • Minipreps - CRISPR library
  • Cultures - CRISPR library (round 2)



Minipreps - CRISPR library


  • Minipreps
    • Sigma GenElute kit, elute w/ 75 μL elution sln.
  1. L34-032
  2. L34-040
  3. L34-094
  4. L34-096


  • Measure conc.'s
Sample OD260 260/280 ng/μL
1. L34-032 0.167 1.863 167.3
2. L34-040 0.026 1.908 26.4
3. L34-094 0.032 1.901 32.4
4. L34-096 0.142 1.859 142.4
  • Sequencing: Order 10437
  1. L34-032, P163
  2. L34-040 "
  3. L34-094 "
  4. L34-096 "



Cultures - CRISPR library (round 2)