User:Karmella Haynes/Notebook/BioBrick cloning/2015/10/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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| Sample || OD260 || 260/280 || ng/μL | | Sample || OD260 || 260/280 || ng/μL | ||
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| 1. MV11 (S/N) || | | 1. MV11 (S/N) || 0.024 || 1.746 || 24.15 | ||
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| 2. H3-1-40 PCR || 0.03 || 1.879 || 29.8 [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/BioBrick_cloning/2015/06/23 06/23/15] | | 2. H3-1-40 PCR || 0.03 || 1.879 || 29.8 [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/BioBrick_cloning/2015/06/23 06/23/15] | ||
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* Ligations | * Ligations (10/12/15) | ||
** > 2:1 ratio calculations... | ** > 2:1 ratio calculations... | ||
** 433 bp largest insert / 10305 bp vector * 2 * 50 ng vector = 4.2 ng | ** 433 bp largest insert / 10305 bp vector * 2 * 50 ng vector = 4.2 ng | ||
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| Insert DNA || 0.5 || --- | | Insert DNA || 0.5 || --- | ||
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| Vector DNA || | | Vector DNA || 2.0 || 2.0 | ||
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| 2x lgn buf (Roche) || 5.0 || 5.0 | | 2x lgn buf (Roche) || 5.0 || 5.0 | ||
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| T4 ligase (NEB) || 1.0 || 1.0 | | T4 ligase (NEB) || 1.0 || 1.0 | ||
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| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 1.5 || 2.0 | ||
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| || 10.0 μL || 10.0 μL | | || 10.0 μL || 10.0 μL | ||
|} | |} | ||
RESULTS (10/ | RESULTS (10/13/15) | ||
* tba | * tba | ||
Latest revision as of 01:14, 27 September 2017
Karmella's BioBrick Cloning | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10/09/15
Cas-tone Project
RESULTS (10/13/15)
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