User:Karmella Haynes/Notebook/BioBrick cloning/Entry Base: Difference between revisions
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** Specific notes | ** Specific notes | ||
{| {{table}} | {| {{table}} cellspacing="3" <!-- Digest rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
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> Measure conc.'s | > Measure conc.'s | ||
{| {{ | {| {{table}} cellspacing="3" <!-- [DNA] table --> | ||
|- | |- | ||
| bgcolor=#cfcfcf | Sample | | bgcolor=#cfcfcf | Sample | ||
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> Dephosphorylation (Roche) | > Dephosphorylation (Roche) | ||
{| | {| {{table}} cellspacing="3" <!-- Dephos table --> | ||
|- | |- | ||
| | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | |||
|- | |- | ||
| DNA (clean digest) || up to 17 μL (500 ng) | | DNA (clean digest) || up to 17 μL (500 ng) | ||
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> Ligations | > Ligations | ||
{| | {| {{table}} cellspacing="3" <!-- Ligations table --> | ||
| | |- bgcolor=#cfcfcf | ||
| Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font> | |||
|- | |- | ||
| 1. insert(a/b)/size, ## ng + vector(c/d)/size, ## ng || <font color="blue">new BioBrick #:1 (Pick #)</font> | | 1. insert(a/b)/size, ## ng + vector(c/d)/size, ## ng || <font color="blue">new BioBrick #:1 (Pick #)</font> | ||
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|} | |} | ||
{| | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
| || 1 || 2 || | | || 1 || 2 || | ||
|- | |- |
Revision as of 14:31, 1 December 2012
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
mm/dd/yy
Minipreps
Assemblies
Oligo annealing
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