User:Karmella Haynes/Notebook/BioBrick cloning/Entry Base: Difference between revisions
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--> Specific notes<br> | --> Specific notes<br> | ||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | ||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> | |||
| rowspan="7" | <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br> | |||
| rowspan="7" | <!-- Gel image [[Image:KAH_111909_gel1.jpg|400px|E/P Digest1 11/19/09]] --> | |||
|- | |- | ||
| | | DNA(plasmid) || 2.0 μL | ||
|- | |- | ||
| | | 10X buffer || 1.5 | ||
|- | |- | ||
| | | EcoRI || 1.0 | ||
|- | |- | ||
| | | PstI || 1.0 | ||
|- | |- | ||
| | | dH<sub>2</sub>O || 9.5 | ||
|- | |- | ||
| || 15 μL --> 37°C/ ~15 min. | |||
| || | |||
|} | |} | ||
Revision as of 07:37, 20 November 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/18/09
Assemblies
> Digests (Fermentas FD)
Oligo annealing
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