User:Karmella Haynes/Notebook/PcTF Genomics

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(PcTF Expression/ Reporter Activation)
(Cell Type Study)
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< [http://openwetware.org/wiki/Haynes_Lab Haynes Lab OWW Home]
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* Fasta --> BAM files: Mark Teng from the Dinu lab (TGEN)
* Fasta --> BAM files: Mark Teng from the Dinu lab (TGEN)
* Enrichment analysis (DNASTAR software)
* Enrichment analysis (DNASTAR software)
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** BAM --> peaks/ enrichment histograms workflow [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2012/12/13 12/13/2012]
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** DNASTAR: BAM --> peaks/ enrichment histograms workflow [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2012/12/13 12/13/2012]
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** BAM --> wig files for UCSC browser workflow [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/01/07 01/07/2013]
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** DNASTAR: BAM --> wig files for UCSC browser workflow [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/01/07 01/07/2013]
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** STAR: Sample BED vs. Input BED --> clean up false positive enrichment and filter out repeat-sequence hits [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2014/02/10 02/10/2014]
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==Cell Type Study==
==Cell Type Study==
* Carly Hom - Express PcTF (KAH126) in K-562 and SK-N-SH
* Carly Hom - Express PcTF (KAH126) in K-562 and SK-N-SH
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** K562 - TRIzol prep [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2012/12/18 12/18/12]
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** SK-N-SH - TRIzol prep [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2012/12/27 12/27/12]
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** U2OS TriZOL prep [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2014/01/27 01/27/14]
==PcTF-Reporter Activation==
==PcTF-Reporter Activation==
* Measure range of PcTF expression in transiently transfected cells - flow cytometry, [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/06/02 06/02/13]
* Measure range of PcTF expression in transiently transfected cells - flow cytometry, [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/06/02 06/02/13]
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==Synthetic Repression==
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* Gal4-EED/luc staining experiments
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Revision as of 18:39, 10 February 2014

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Illumina ChIP-seq

  • ChIP DNA prep for Illumina:
  • Fasta --> BAM files: Mark Teng from the Dinu lab (TGEN)
  • Enrichment analysis (DNASTAR software)
    • DNASTAR: BAM --> peaks/ enrichment histograms workflow 12/13/2012
    • DNASTAR: BAM --> wig files for UCSC browser workflow 01/07/2013
    • STAR: Sample BED vs. Input BED --> clean up false positive enrichment and filter out repeat-sequence hits 02/10/2014



Cell Type Study

  • Carly Hom - Express PcTF (KAH126) in K-562 and SK-N-SH

PcTF-Reporter Activation

  • Measure range of PcTF expression in transiently transfected cells - flow cytometry, 06/02/13


Synthetic Repression

  • Gal4-EED/luc staining experiments


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